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Hurlin
Lab
 Research Interest
My
research in the Hurlin lab is focused on the ability of mutant
activated fibroblast growth factor receptors (FGFRs) to induce stem
cell-like properties in cultured cells. Both cMyc mRNA and protein
are strongly upregulated by ectopic expression of mutant activated
FGFR in 10T1/2 cells.
Histone deacetylase 6 (HDAC6) has been shown to regulate cMyc
transcription through beta-catenin upon EGF stimulation (Yu Li et
al.), and also a number of transcription factors are phosphorylated
within 10 minutes of FGF2 stimulation.
These include Erf, Stat3 and Ets1, which have all been shown
to regulate cMyc transcription.
Characterization of up-regulation of cMyc in mutant activated
FGFR2 has been carried out through chromatin immunoprecipitation
experiments.
BIOGRAPHICAL
SKETCH
Name:
Chia-Yi (Connie)
Shung
Role on project: Postdoctoral Fellow
Institution
& Location
Degree
Year(s) Field of
Study
The Providence University,
Taichung, Taiwan B.S. 1996-2000
Nutrition
The University of
Texas at San Antonio
M.S. 2001-2003 Biology
The University of Texas at San Antonio
Ph.D. 2003-2007 Cell and
Molecular Biology
Research
and/or Professional Experience:
I earned my Ph.D. in biology with an emphasis in cell and molecular
biology from the University
of Texas at San
Antonio in July 2007. My dissertation
research was focused on Geminiviruses transcriptional and
post-transcriptional regulation. The projects involved mapping
promoter elements for viral transcription, demonstrating that early
viral proteins need to be repressed in order to turn on downstream
gene expression, and upstream AUGs play an inhibitory role in
regulating viral protein expression. I have also initiated a
new project, which involves characterization of a putative viral
protein, which is conserved among the geminiviruses.
Within the duration of my graduate school studies in Dr. Garry
Sunter’s laboratory, I have acquired a significant number of
molecular techniques such as DNA cloning, DNA/RNA isolation,
purification, Southern blot, Northern blot,
in vitro transcription,
In vitro translation,
SDS-PAGE, Western blot, Immunoprecipitation, Insect, plant
suspension cell culture, RNase protection assays, primer extension,
circularization RT-PCR, Clustalx, MEGA, Affymetrix GeneChip, and
real time RT-PCR.
Publications
List
Chia-Yi Shung and Garry
Sunter. AL1-dependent repression of transcription enhances
expression of Tomato golden mosaic virus AL2 and AL3.
Virology. Vol. 364, 2007, pp. 112-122
Chia-Yi Shung, Janet Sunter, Shyam S.
Sirasanagandla and Garry Sunter.
Distinct viral sequence elements are necessary for expression
of Tomato golden mosaic virus complementary sense transcripts that
direct AL2 and AL3 gene expression.
Molecular plant- microbe interaction. Vol. 19, No. 12, 2006,
pp.1394- 1405
Hui Wang, Linhui Hao, Chia-Yi Shung, Garry Sunter,
and David M. Bisaro.
Adenosine kinase is inactivated by Geminivirus AL2 and L2 proteins.
Plant cell. Vol. 15,
2003, pp.3020-3032
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