MicroscopyConfocal laser scanning microscopy (CLSM or LSCM) is a valuable tool for obtaining high resolution images and 3-D reconstructions. The key feature of confocal microscopy is its ability to produce blur-free images of thick specimens at various depths. The principle for this special kind of microscopy was developed by Marvin Minsky in 1953, but it took another thirty years and the development of lasers as near-ideal point light sources for confocal microscopy to become a standard technique toward the end of the 1980s. The Research Center is proud to be able to have available its own Confocal Microscope and below you will find some of the exciting and extremely valuable images we have been able to capture.
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Cos-7 cell expressing Fgfr3-GFP (Fibroblast growth factor receptor-3 - Green Fluorescent Protein) fusion protein (green) and also stained with LysoTracker-Red (red), which localizes to lysosomes. Both occupy round vesicles. Yellow suggests that Fgfr3-GFP resides in lysosomes. |
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Cos-7 cell expressing both Fgfr3-GFP (Fibroblast growth factor receptor-3 - Green Fluorescent Protein) fusion protein (green) and Sprouty 2- DsRed (red). Fgfr3-GFP resides in small vesicles throughout the cells while Sprouty 2 is located mainly near the center of the cell where it co-localizes substantially with Fgfr3-GFP (yellow). |
Cos-7 cell expressing both Fgfr3-GFP (Fibroblast growth factor receptor-3 - Green Fluorescent Protein) fusion protein (green) and c-Cbl-DsRed (red). Fgfr3-GFP resides in small vesicles in central region of cell. c-Cbl-DsRed is distrubted diffusely throughout the cell and especially in distinct, but poorly-defined cytoplasmic structures. There is little co-localization of Fgfr3-GFP and c-Cbl-DsRed. |
Movie expressing both Fgfr3-GFP and Sprouty 2- DsRed: Note subtle movement of Fgfr3-GFP-containing vesicles.
Movie expressing both Fgfr3-GFP and c-Cbl-DsRed: Note movement of Fgfr3-GFP-containing vesicles and fusion and break-up of c-Cbl-DsRed-containing structures.