HPLC Separation System

The Facility has 2 Agilent 1090 HPLC systems for purification
and peptide separations. These systems can be used as analytical
instruments for peptide mapping or as semi-preparative systems for
other analyses. When internal sequence analysis is needed on proteins,
the samples are digested with a protease or chemically cleaved to
generate smaller peptides. These are then separated on a choice
of reverse phase, ion-exchange, or size-exclusion columns and
fractions collected. Individual fractions are applied to the
Procise Sequencer for sequence determination.