6^th Pan Pacific Connective Tissue Societies Symposium

Hilton Waikoloa Village, Hawaii

November 30, 2005 to December 5, 2005

Wednesday, November 30, 2005

6:00 - 9:00 6^th PPCTS Symposium Dinner honoring Erkki Ruoslahti chaired by Teruo Nishida, Japan and Hans Peter Bächinger, USA.

Thursday, December 1, 2005

8:30 - 10:30 Session 1  Cell-Matrix Interactions

Reinhard Fässler, Germany and Mina Bissell, USA

8:30 - 8:45            Zena Werb introduces Mina Bissell

8:45 - 9:30            Mina Bissell “tba”

9:30 - 10:00            Reinhard Faessler “Integrin-fibronectin interaction in vivo and in vitro”

10:00 - 10:15            Anita Woods “ROCK signaling and actin dynamics control growth plate physiology”

10:15 - 10:30            Noriko Akimoto “Involvement of TIMP-2 in sebaceous gland development and differentiation in vivo and in vitro”

10:30 - 11:00 Coffee Break

11:00 - 12:30 Session 2  Matricellular Proteins

Karen Lyons, USA and Helene Sage, USA

11:00 - 11:30            Helene Sage “Matricellular hevin: Where collagen assembly and fibroblast migration meet”

11:30 - 12:00            Karen Lyons “Roles of CCN proteins in connective tissues”

12:00 - 12:30            Takako Sasaki “Mapping of the BM-40/SPARC/osteonectin-binding epitope on collagen III”

3:00 - 5:00 Poster Session 1

7:00 - 8:20 Session 3  Heritable disorders of connective tissue

Peter Byers, USA and Lynn Sakai, USA

7:00 - 7:15            Rick Wenstrup introduces Peter Byers

7:15 - 7:45            Peter Byers “Clinical and molecular genetics of type I collagen genes”

7:45 - 8:15            Dan Judge “TGF-β antagonism in mouse models of Marfan Syndrome”

8:15 - 8:30            Alana Majors “Improving the structure of the extracellular matrix produced by aortic smooth muscle cells from patients with Marfan Syndrome”

8:30 - 8:45            Jacqueline Hecht “COMP mutations, chondrocyte function and cartilage matrix”

8:45 - 10:15 Session 4  Skeletal muscle and neuromuscular junction

Shireen Lamandé, Australia and Eri Arikawa-Hirasawa, Japan

8:45 - 9:00            Lynn Sakai introduces Eva Engvall

9:00 - 9:30            Shireen Lamandé “The collagen VI muscular dystrophies: Correlating disease severity with the collagen VI assembly pathway”

9:30 - 10:00            Eri Arikawa-Hirasawa “Role of perlecan in skeletal and smooth muscle contraction”

10:00 - 10:15            Takanori Nishimura “Expression of decorin in muscle satellite cells”

Friday, December 2, 2005

8:30 - 10:15 Session 5  Growth Factors (TGFβ and BMPs)

Dan Rifkin, USA and Kohei Miyazono, Japan

8:30 - 9:00            Dan Rifkin “Latent TGF-β binding protein (LTBP): An isoform specific modulator of TGF-β activity in vivo and in vitro”

9:00 - 9:30            Kohei Miyazono “TGF-β signaling: Mechanisms of regulation”

9:30 - 9:55            Gerhard Sengle “Regulation of BMPs by fibrillin”

9:55 - 10:15            Kaori Ihida-Stansbury “Mutated bone morphogenetic protein type II receptors induce expression of tenascin-C by down-regulation or R-Smad signaling via ERK1/2 MAPK”

10:15 - 10:45 Coffee Break

10:45 - 12:15 Session 6  Growth Factors (FGFs)

Matt Hoffman, USA and William Horton, USA

10:45 - 11:15            Matt Hoffman “FGF10 function during submandibular gland branching morphogenesis”

11:15 - 11:45            William Horton “Modulation of FGFR3 signals during linear bone growth”

11:45 - 12:00            Frederic Mercier “Brain neurogenic zone basal laminae (fractones) sequester FGF-2: A heparin-binding mechanism for orchestrating the adult neural stem cell niche?”

12:00 - 12:15            Melinda Larsen “Epithelial cell dynamics during branching morphogenesis”

Afternoon Snorkel/Sail

7:00 - 7:45 Session 7  Proteomics

Ruedi Aebersold, USA/Switzerland

7:00 - 7:45            Ruedi Aebersold “Quantitative proteomics and systems biology”

7:45 - 9:45 Session 8  Translational Research

David Eyre, USA and Yukihide Iwamoto, Japan

7:45 - 8:00            Hans Peter Bächinger introduces David Eyre

8:00 - 8:30            David Eyre “Collagen degradation products as biomarkers of cartilage metabolism and joint disease”

8:30 - 9:00            Yukihide Iwamoto “Mechanisms and Treatments of Bone Metastais”

9:00 - 9:15            Akira Ito “Triptolide, a diterpenoid triepoxide, from Tripterygium wilfordii Hook. f. suppresses both joint inflammation and cartilage destruction in collagen-induced arthritis in mice”

9:15 - 9:30            Takao Iwai “Low-intensity pulsed ultrasound and new bone formation within interconnected porous calcium hydroxyapatite ceramics”

9:30 - 9:45            Hui Xu “Remodeling of an acellular extracellular matrix scaffold in a primate model of abdominal wall repair”

Saturday, December 3, 2005

8:30 - 10:15 Session 9  Proteoglycans

Jeffrey Esko, USA and Koji Kimata, Japan

8:30 - 9:00            Jeffrey Esko “Hepatic clearance of triglyceride-rich lipoproteins depends on heparan sulfate”

9:00 - 9:30            Sonoko Hatano “Versican/PG-M is essential for mice cardiovascular and dermal development”

9:30 - 9:45            Pyong Woo Park “Syndecan-1 attenuates inflammatory multi-organ injury and dysfunction in systemic inflammatory response syndromes (SIRS)”

9:45 - 10:00            Zenzo Isogai “Versican bound to microfibrils in connective tissue aging”

10:00 - 10:15            Kayoko Oguri “Cell surface heparin sulfate proteoglycan functions as a receptor of MMP-9”

10:15 - 10:45 Coffee Break

10:45 - 12:30 Session 10  Cartilage Development and Engineering

Rocky Tuan, USA and Tomoatsu Kimura, Japan

10:45 - 11:15            Rocky Tuan “Skeletal tissue engineering and regeneration: Cells, scaffold, and biology”

11:15 - 11:45            Tomoatsu Kimura “Cartilage repair using intra-articular injection of mesenchymal cells”

11:45 - 12:00            Jamie Fitzgerald “WARP is a novel multimeric component of the chondrocyte pericellular matrix that interacts with perlecan”

12:00 - 12:15            Veronica Ulici “PI3K/Akt pathway regulates endochondral bone growth”

12:15 - 12:30            Nicolai Miosge “Chondrogenic progenitor cells derived from late stages of human osteoarthritis”

2:00 - 4:00 Poster Session 2

4:00 - 5:00 Business Meeting

7:00 - 8:45 Session 11  Basement Membranes

Yoshifumi Ninomiya, Japan and Uli Mayer, UK

7:00 - 7:30            Yoshifumi Ninomiya “Capillary basement membranes and their functions in the central nervous system”

7:30 - 8:00            Uli Mayer “Distinctive properties of the nidogen binding site of the laminin γ1 chain”

8:00 - 8:15            Jennifer Koblinski “A peptide, AG73, from the laminin-a1 chain increases breast cancer metastasis to bone and brain”

8:15 - 8:30            Yuki Ogura “Basement Membrane damage induced by plasmin: Dysfunction of degraded laminin-5”

8:30 - 8:45            Ken-ichi Iyama “The expression of type IV collagen a5 and a6 chains is silenced in colorectal cancer cells with the hypermethylation of their common promoter region”

8:45 - 10:15 Session 12  Transcription Factors in Development of Connective Tissues

Scott Stadler, USA and Yoshi Yamada, USA

8:45 - 9:15            Yoshi Yamada “Epiprofin, a zinc-finger transcription factor, is essential for tooth and hair development”

9:15 - 9:45            Scott Stadler “HOX-dependent regulation of BMP signaling””

9:45 - 10:00            George Bou-Gharios “Transcriptional regulation of COL1A2”

10:00 - 10:15            Chisa Shukunami “Tenomodulin, a marker for tenocytes, is positively regulated by the basic helix-loop-helix transcription factor, Scleraxis”

Sunday, December 4, 2005

8:30 - 10:15 Session 13  MMPs and ADAMS

Zena Werb, USA and Yasunori Okada, Japan

8:30 - 9:00            Zena Werb “The regulation of mammary gland morphogenesis by metalloproteinases”

9:00 - 9:30            Satsuki Mochizuki “Expression of ADAM28 is implicated in human breast carcinoma cell proliferation”

9:30 - 9:45            John Fessler “New ECM-metalloprotease interactions during development”

9:45 - 10:00            Jennifer Cox “Regulation of inflammatory and immune responses by matrix metalloproteinase processing of CXCR3 chemokines”

10:00 - 10:15            Hiroyuki Ishida “Prevention of cancer cell invasion by inhibiting the binding of MMP-1 to extracellular matrix metalloproteinase inducer (EMMPRIN)”

10:15 - 10:45 Coffee Break

10:45 - 12:30 Session 14  Biosynthesis of extracellular matrix molecules

Kazuhiro Nagata, Japan and Hans Peter Bächinger, USA

10:45 - 11:15            Kazuhiro Nagata “Role of collagen-specific molecular chaperone HSP47 in the correct folding and fibril formation of procollagens; lessons from mice and cells disrupted with hsp47 gene”

11:15 - 11:45            Janice Vranka “Patterns of expression of the prolyl 3-hydroxylase family members in mouse tissues”

11:45 - 12:00            Toshihiko Hayashi “Accumulation and condensation of type V collagen fibrils in the intercellular space of cultured human fibroblasts”

12:00 - 12:15            Keisuke Tanaka “Insufficient type I collagen deposition by Juvenile hyaline fibromatosis derived cells”

7:00 - 11:00 Tropical Dinner

Monday, December 5, 2005

8:30 - 10:15 Session 15  Structure of extracellular matrix molecules

Kenji Okuyama, Japan and Erhard Hohenester, UK

8:30 - 9:00            Erhard Hohenester “Functional dissection of the guidance molecule Slit”

9:00 - 9:30            Kenji Okuyama “Revision of collagen molecular structure”

9:30 - 9:45            Yasutada Imamura “Atomic force microscopic observation of type V collagen molecules”

9:45 - 10:00            Andrzej Fertala “Some collagen II mutants alter the formation of ECM as a result of atypical interaction with fibronectin”

10:00 - 10:15            Kazunori Mizuno “Structural property of 3-hydroxyproline in the collagen helix”

10:15 - 10:45 Coffee Break

10:45 - 12:30 Session 16  Matrix macromolecules

Kathy Cheah, Hongkong and Lynn Sakai, USA

10:45 - 11:15            Kathy Cheah “The role of procollagen IIA as a facilitator of BMP signaling”

11:15 - 11:35            Rick Wenstrup “tba”

11:35 - 11:55            Elaine Davis “Fibulin-5 null mice reveal that fibulin-5 is essential for cutaneous elastic fiber assembly and the mechanical property of skin”

11:55 - 12:15            Fred Keeley “Phylogenetic insights into the evolutionary history and structure/function of elastin”

12:15 - 12:30            Anders Schlosser “Characterization of mouse microfibril-associated protein 4 (mMFAP4)”

2:00 - 3:15 Session 17  Skin

Pete Marinkovich, USA and Satoshi Amano, Japan

2:00 - 2:30            Pete Marinkovich “Role of Laminin-10 in hair development

2:30 - 3:00            Satoshi Amano “Time course analyses of expression and restructuring of elastic fiber components in grafted skin-equivalent models”

3:00 - 3:15            Concluding remarks